微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 172-12A4 |
|---|---|
| 商品货号： | TS213011 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic peptide with the sequence LGSGAFGTIYKG(C) corresponding to residues 138-149 of the v-erb B oncogene. The 170 kd EGF receptor is weakly detected in immunoblot screening of A431 cells. In addition, a protein of approximately 100 kd is detected in extracts of human brain. |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic v-erb B oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic v-erb B oncogene peptide |
| Comments： | Animals were immunized with a synthetic peptide with the sequence LGSGAFGTIYKG(C) corresponding to residues 138-149 of the v-erb B oncogene. Spleen cells were fused with Sp2/0 myeloma cells. In ELISA tests using synthetic peptides representing erb B, fes/fps, fgr, fms, src/yes, mil/raf, abl, mos, CDC28 and the catalytic and regulatory subunit of cyclic AMP dependent protein kinase, the antibody recognizes only the erb B sequence. The 170 kd EGF receptor is weakly detected in immunoblot screening of A431 cells. In addition, a protein of approximately 100 kd is detected in extracts of human brain. The IgG1 and IgG2b reactivities of the antibody with the v-erb B peptide (138 to 149) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 and IgG2b |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |