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微信小章| 产品名称： | 184B5 |
|---|---|
| 商品货号： | TS213015 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland/breast: epithelium |
| Cell Type： | chemically transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 21 years |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype： | 47, XX |
| Derivation： | Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established.xa0The 184B5 cell line was established from normal mammary tissue obtained from a normal reduction mammoplasty. |
| Clinical Data： | female |
| Tumorigenic： | No |
| Effects： | No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | The line appears to be immortal, but is not malignant. When seeded at low density, the cells grow in tightly packed colonies. |
| Complete Growth Medium： | The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit.
To make the complete growth medium, you will need to add the following components to the kit (sold separately):
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium suplemented with 10% (v/v) fetal bovine serum and 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Milo G, et al. (eds.)., Transformation of human epithelial cells. Boca Raton, FL: CRC Press; 1992. Stampfer MR. Continuous human cell lines and method of making same. US Patent 4,808,532 dated Feb 28 1989 Walen KH, Stampfer MR. Chromosome analyses of human mammary epithelial cells at stages of chemical-induced transformation progression to immortality. Cancer Genet. Cytogenet. 37: 249-261, 1989. PubMed: 2702624 Stampfer MR, Bartley JC. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzoapyrene. Proc. Natl. Acad. Sci. USA 82: 2394-2398, 1985. PubMed: 3857588 |