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| 产品名称： | 162-46.2 Trop-2 |
|---|---|
| 商品货号： | TS213020 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Choriocarcinoma |
| Derivation： | Animals were immunized with BeWo cells (ATCC CCL-98). Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the trop-2 antigen of human trophoblasts cells and choriocarcinomas |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the trop-2 antigen of human trophoblasts cells and choriocarcinomas |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | The antibody defines an antigen that is expressed on trophoblasts and choriocarcinoma cells but not on blood cells or other tumor cells.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. |
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium:xa0Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature:xa0Liquid nitrogen vapor phase |
| Isotype： | IgG1 (Igh-4a allotype) |
| Name of Depositor： | AM Stall |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529 |