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微信小章| 产品名称： | 16H3 |
|---|---|
| 商品货号： | TS213025 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with approximately 25 proteins containing the "alternating arginine" motif shared by RNA processing factors. It also binds to a conserved epitope ("alternating arginine") present on approximately 25 nuclear antigens, many of which are involved in pre-mRNA processing. The antibody binds to nuclear proteins in human, frog and Drosophila extracts. It can be used to determine whether a factor is involved in pre-mRNA processing; also used in isolation or depletion of RNA processing activate. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified dephosphorylated bovine SRp55 and subsequently with dSRp55-GST. Spleen cells were fused with FOX-NY myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a conserved epitope on a subset of SR proteins. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a conserved epitope on a subset of SR proteins. |
| Comments： | Animals were immunized with purified dephosphorylated bovine SRp55 and subsequently with dSRp55-GST. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with approximately 25 proteins containing the "alternating arginine" motif shared by RNA processing factors. These include four of the family of six SR proteins (SRp20, SRp40, SRp55 and SRp75), U2AF65, U2AF35, HRH1, and U17OK. It also binds to a conserved epitope ("alternating arginine") present on approximately 25 nuclear antigens, many of which are involved in pre-mRNA processing. The epitope has been defined as containing arginine alternating with glutamate, asparate, or-phospho-serine. The antibody binds to nuclear proteins in human, frog and Drosophila extracts. It can be used to determine whether a factor is involved in pre-mRNA processing; also used in isolation or depletion of RNA processing activate. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal:xa0 Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | KM Neugebauer, MB Roth |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Neugebauer KM, et al. A conserved epitope on a subset of SR proteins defines a larger family of Pre-mRNA splicing factors. J. Cell Biol. 129: 899-908, 1995. PubMed: 7538140 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |