微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 151-5-G2-12 CO-512, CO512 |
|---|---|
| 商品货号： | TS213030 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human Lewis a and Lewis b blood group antigens |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human Lewis a and Lewis b blood group antigens |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | 50% FBS+ 40%Culture Medium+10% DMSO |
| Isotype： | IgG3 |
| Name of Depositor： | M Herlyn |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Blaszczyk M, et al. Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch. Biochem. Biophys. 233: 161-168, 1984. PubMed: 6465894 |