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微信小章| 产品名称： | 142-24E5 1/24E05, 142-24E05, SCRF 35.1 |
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| 商品货号： | TS213039 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension but many cells attach |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic polypeptide with the sequence YREQIKRVKDSDDVPMVLVGNKC corresponding to peptides 96 to 118 of the predicted p21 sequence of the ras (Ha-ras) oncogene, formerly v-ras (H). The antibody reacts in indirect fluorescence assays of frozen sections and peroxidase stain paraffin embedded cell lines or tissues. |
| Images： |  |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic H/N-ras oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic H/N-ras oncogene peptide |
| Comments： | Animals were immunized with a synthetic polypeptide with the sequence YREQIKRVKDSDDVPMVLVGNKC corresponding to peptides 96 to 118 of the predicted p21 sequence of the ras (Ha-ras) oncogene, formerly v-ras (H). Spleen cells were fused with Sp2/0 myeloma cells. The antibody recognizes a 23,000 dalton protein in all mammalian cell lines tested, a 38,000 dalton in yeast cells, and proteins of 100,000, 55,000 and 23,000 daltons in the urine of a variety of individuals. The antibody reacts in indirect fluorescence assays of frozen sections and peroxidase stain paraffin embedded cell lines or tissues. A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 5 X 10 exp4 and 1 to 2 X 10 exp6 viable cells/ml. |
| Cryopreservation： | Culture medium with 45% fetal bovine serum, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991 Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,030,565 dated Jul 9 1991 Chesa PG, et al. Expression of p21ras in normal and malignant human tissues: lack of association with proliferation and malignancy. Proc. Natl. Acad. Sci. USA 84: 3234-3248, 1984. PubMed: 3554234 Habib NA, Wood CB. The expression of ras oncogene in normal and pathological liver disease. Int. Surg. 71: 182-183, 1986. PubMed: 3771121 Bokoch GM, Parkos CA. Identification of novel GTP-binding proteins in the human neutrophil. FEBS Lett. 227: 66-70, 1988. PubMed: 3123273 Luo JC, et al. Expression p21 ras-related protein in the plasma and tissue of patients with adenomas and carcinomas of the colon. Biomarkers 1: 29-33, 1996. |