微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 143B PML BK TK |
|---|---|
| 商品货号： | TS213041 |
| Organism： | Homo sapiens, human |
| Tissue： | bone |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Age： | 13 year old |
| Gender： | female |
| Ethnicity： | Caucasian |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data： | female Caucasian 13 year old |
| Genes Expressed： | genome, partial tk |
| Comments： | Contains a thymidine kinase (TK+) plasmid (PMBKTK). |
| Complete Growth Medium： | Minimum essential medium (Eagle) in Earles BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 11 vWA: 18 |
| Name of Depositor： | Wistar Institute |
| U.S. Patent Number： |