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微信小章| 产品名称： | 143B |
|---|---|
| 商品货号： | TS213042 |
| Organism： | Homo sapiens, human |
| Tissue： | bone |
| Product Format： | frozen |
| Morphology： | mixed |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Age： | 13 year old |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data： | 13 year old Caucasian female |
| Comments： | Thymidine kinase negative (TK-). This is a human osteosarcoma cell line. |
| Complete Growth Medium： | Minimum essential medium (Eagle) in Earles BSS with 0.015 mg/ml 5-bromo-2-deoxyuridine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37°C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | 90% FBS; 10% DMSO |
| Culture Conditions： | Temperature: 37°C
|
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 11 vWA: 18 |
| Name of Depositor： | Wistar Institute |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758 Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Lebers hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 8662757 Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651 |