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微信小章| 产品名称： | 145-2C11 |
|---|---|
| 商品货号： | TS213046 |
| Organism： | Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Tissue： | Spleen, blood |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor. The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb).xa0Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse CD3 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse CD3 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | IgG , IgG |
| Name of Depositor： | JA Bluestone |
| Deposited As： | hamster, Armenian (B cell); mouse (myeloma) |
| References： | Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524 Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079 |