Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Using immunoblot techniques, the ascites produced from these cells recognized proteins of approximately 45,000 and 75,000 daltons in a variety of transformed mammalian cells.

In addition, proteins of 45,000 and 55,000 were detected in various urine samples.

Animals were immunized with a synthetic polypeptide with the sequence LGEHHCTPSPPVDHG corresponding to peptides 348 to 363 of the c-myb oncogene, formerly v-myb (residues 160 to 175).

Animals were immunized with a synthetic polypeptide with the sequence LGEHHCTPSPPVDHG corresponding to peptides 348 to 363 of the c-myb oncogene, formerly v-myb (residues 160 to 175). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the ascites produced from these cells recognized proteins of approximately 45,000 and 75,000 daltons in a variety of transformed mammalian cells. In addition, proteins of 45,000 and 55,000 were detected in various urine samples. The IgG1 and IgG2b reactivities of the antibody with the c-myb peptide (348 to 363) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive. A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

immunoglobulin; monoclonal antibody; against a synthetic c-myb oncogene peptide

immunoglobulin; monoclonal antibody; against a synthetic c-myb oncogene peptide

Yes, produces ascites in female 129G1X+ x BALB/c mice

Animals were immunized with a synthetic polypeptide with the sequence LGEHHCTPSPPVDHG corresponding to peptides 348 to 363 of the c-myb oncogene, formerly v-myb (residues 160 to 175). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the ascites produced from these cells recognized proteins of approximately 45,000 and 75,000 daltons in a variety of transformed mammalian cells. In addition, proteins of 45,000 and 55,000 were detected in various urine samples. The IgG1 and IgG2b reactivities of the antibody with the c-myb peptide (348 to 363) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive. A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C