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| 产品名称： | 123-10 |
|---|---|
| 商品货号： | TS213061 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against DNA polymerase III holoenzyme from Escherichia coli (E. coli) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against DNA polymerase III holoenzyme from Escherichia coli (E. coli) |
| Comments： | Mice were immunized with E. coli DNA polymerase III containing the alpha, epsilon, theta and tau subunits. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 85%; fetal bovine serum, 15%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgM |
| Name of Depositor： | M Franden, CS McHenry |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Wu YH, et al. Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme. J. Biol. Chem. 259: 12117-12122, 1984. PubMed: 6090455 |