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微信小章| 产品名称： | 11B11 BSF-1 |
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| 商品货号： | TS213070 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody does not cross-react with other lymphokines, and can be used to purify BSF-1 from complex mixtures.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified BSF-1 emulsified in complete Freunds adjuvant. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1) |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106xa0cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | 70% Culture Media + 20% FBS + 10% DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Isotype： | IgG1 |
| Name of Depositor： | WE Paul |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |