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| 产品名称： | 11P0-1 |
|---|---|
| 商品货号： | TS213075 |
| Organism： | Mus musculus, mouse |
| Cell Type： | mast cell; Abelson murine leukemia virus transforme |
| Product Format： | frozen |
| Morphology： | mast cell |
| Culture Properties： | suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 11 days gestation |
| Applications： | The 11P0-1 cell line was established by transformation of placental cells from an 11 day mouse embryo with A-MuLV. |
| Derivation： | The 11P0-1 cell line was established by transformation of placental cells from an 11 day mouse embryo with A-MuLV. |
| Receptor Expression： | FcERI (Fc of IgE) |
| Comments： | The 11P0-1 cell line was established by transformation of placental cells from an 11 day mouse embryo with A-MuLV. The cells are surface immunoglobulin positive, B23.1 positive, B220 positive and possess receptors for IgE. This line represents a transformed stage of a very early hematopoietic precursor. |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp4 cells/ml and maintain between 1 X 10 exp4 and 5 X 10 exp5 cells/ml. Do not allow the cell density to exceed 5 X 10 exp5 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | EJ Siden |
| Deposited As： | Mus musculus |
| References： | Siegel ML, et al. Isolation and characterization of Abelson murine leukemia virus- transformed mast cell lines from midgestation embryonic placenta. Eur. J. Immunol. 15: 1136-1141, 1985. PubMed: 2998805 Siden EJ. Regulated expression of germline antigen receptor genes in mast cell lines from the murine embryo. J. Immunol. 150: 4427-4437, 1993. PubMed: 7683318 |