微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 11F11 |
|---|---|
| 商品货号： | TS213077 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933. The antibody reacts with the A subunit of SLT-II and does not react with B subunit. The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains. It may also be used to differentiate SLT-II from SLT-I. |
| Derivation： | Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II) |
| Comments： | Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the A subunit of SLT-II and does not react with B subunit. The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains. It may also be used to differentiate SLT-II from SLT-I. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | AD OBrien |
| References： | Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26: 2127-2131, 1988. PubMed: 3053764 |