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微信小章| 产品名称： | 10P12 |
|---|---|
| 商品货号： | TS213086 |
| Organism： | Mus musculus, mouse |
| Cell Type： | mast cell; Abelson murine leukemia virus transformed |
| Product Format： | frozen |
| Morphology： | mast cell |
| Culture Properties： | suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 10 days gestation |
| Applications： | The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV. |
| Receptor Expression： | FcERI (Fc of IgE) |
| Comments： | The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV. The cells are surface immunoglobulin positive, B23.1 positive, B220 positive and possess receptors for IgE. This line represents a transformed stage of a very early hematopoietic precursor and shares surface antigens with multipotent stem cells. Transcripts of germline immunoglobulin and T cell receptor genes are detectable. |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 104 cells/mL and maintain between 1 x 104 and 5 x 105 cells/mL. Do not allow the cell density to exceed 5 x 105 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | EJ Siden |
| Deposited As： | Mus musculus |
| References： | Siegel ML, et al. Isolation and characterization of Abelson murine leukemia virus- transformed mast cell lines from midgestation embryonic placenta. Eur. J. Immunol. 15: 1136-1141, 1985. PubMed: 2998805 Siden EJ. Regulated expression of germline antigen receptor genes in mast cell lines from the murine embryo. J. Immunol. 150: 4427-4437, 1993. PubMed: 7683318 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |