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微信小章| 产品名称： | Chlamydia trachomatis |
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| 商品货号： | TS213205 |
| Classification： | Chlamydiaceae, Chlamydia |
| Deposited As： | Lymphogranuloma venereum |
| Agent： | Chlamydia trachomatis |
| Strain： | Lymphogranuloma venereum (LGV I) strain 440 |
| Applications： | Sexually Transmitted Disease Research |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Centrifuge at 2000 X g for 1 hour; adsorb 1 hour at 35-36°C. Feed with 1.0 mL EMEM (HEPES buffered) 5mg glucose/mL, 0.3 mg glutamine/mL, 1-2 µg/mL cycloheximide. Dilute high titer stocks to avoid cell toxicity.
Tissue culture adapted at ATCC. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
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| Recommended Host： | McCoy cells (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 37°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent.
Incubation: 2-3 days, a 5% CO2 in air atmosphere is recommended
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| Name of Depositor： | J Schachter |
| Source： | Lymph node from human with LGV |
| References： | Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618 Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529 |
| Cross References： | Nucleotide (GenBank) : AJ012175 Chlamydia trachomatis partial rnpB gene for RNase P RNA subunit. |