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| 产品名称： | Human parainfluenza virus 1 |
|---|---|
| 商品货号： | TS213221 |
| Classification： | Paramyxoviridae, Respirovirus |
| Deposited As： | Parainfluenza 1 |
| Agent： | Human parainfluenza virus 1 |
| Strain： | C35 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Addition of 1 μg/mL trypsin (1:250 Gibco trypsin catalog #27250018) to culture medium is required for optimal growth. Agglutinates both chicken and guinea pig RBC (0.5%) but the latter to a higher titer than the former. Based on electron microscopic evidence, this product may also contain an as yet unidentified polyoma virus. Virus presence can be detected with Millipore #5019 Parainfluenza 1 Antibody FITC Reagent |
| Effect on Host： | Slight CPE, rounding and sloughing |
| Recommended Host： | LLC-MK2 cells (ATCC® CCL-7.1x99) |
| Growth Conditions： | Temperature: 37°C
Recommendations For Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-7 days continuously rocking at 37°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | R Huebner, RM Chanock, JC Hierholzer, A Chappell |
| Source： | Throat swab, 3-year-old boy with acute laryngitis, Washington, DC, 1957 |
| References： | Andrewes CH, et al. Para-influenza viruses 1, 2, and 3: Suggested names for recently described myxoviruses. Virology 8: 129-130, 1959. PubMed: 13669331 Chanock RM. . N. Engl. J. Med. 258: 207-213, 1958. |
| Cross References： | Nucleotide (GenBank) : JQ901971.1 Human parainfluenza virus 1 strain TS213221, partial genome |