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| 产品名称： | Rickettsia japonica Uchida et al. |
|---|---|
| 商品货号： | TS213229 |
| Classification： | Rickettsiae |
| Agent： | Rickettsia japonica Uchida et al. |
| Strain： | YH |
| Applications： | Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | blood of patient with oriental spotted fever, Japan |
| Product Format： | frozen |
| Storage Conditions： | Liquid nitrogen freezer |
| Comments： | Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. Vero cells demonstrated to be mycoplasma-free before infection. Infected Vero cells grow like uninfected cells in MEM with 10% FBS devoid of antibiotics. Subculture by dispersion with 0.05% trypsin and 0.02% EDTA is possible for many passages. Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit. |
| Effect on Host： | Cytopathic effects in BHK and L929 No observable CPE in Vero Death of CE Fever and scrotal swelling of guinea pig |
| Recommended Host： | Vero cells (ATCC CCL-81) BHK-21(C-13) cells (ATCC CCL-10); L-929 cells (ATCC CCL-1); chicken embryo; guinea pig |
| Growth Conditions： | Temperature: 34°C Incubation: 3-6 days |
| Name of Depositor： | T Uchida |
| Source： | blood of patient with oriental spotted fever, Japan |
| References： | Uchida T, et al. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42: 303-305, 1992. PubMed: 1581190 Uchida T, et al. Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol. Immunol. 30: 1323-1326, 1986. PubMed: 3553872 type strain |