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| 产品名称： | Human respiratory syncytial virus |
|---|---|
| 商品货号： | TS213285 |
| Classification： | Paramyxoviridae, Pneumovirus |
| Deposited As： | Respiratory syncytial virus |
| Agent： | Human respiratory syncytial virus |
| Strain： | B WV/14617/85 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Grows poorly in Balb/c mice. |
| Effect on Host： | CPE, syncytia, rounding, sloughing |
| Recommended Host： | Vero (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C
Recommendations For Infection:xa0Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum.xa0 End adsorption by adding virus growth medium.
Incubation: 3-9 days
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| Name of Depositor： | JE Crowe, National Institutes of Allergy and Infectious Diseases |
| Source： | Secretion from endotracheal tube of human infant with pneumonia |
| References： | Crowe JE, et al. Live subgroup B respiratory syncytial virus vaccines that are attenuated, genetically stable, and immunogenic in rodents and nonhuman primates.. J. Infect. Dis. 173: 829-839, 1996. PubMed: 8603960 |