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| 产品名称： | Avian coronavirus |
|---|---|
| 商品货号： | TS213297 |
| Classification： | Coronaviridae, Gammacoronavirus |
| Deposited As： | Infectious bronchitis virus |
| Agent： | Avian coronavirus |
| Strain： | Egg-adapted (Michigan State Univ. Repos. Code 42) |
| Common Name： | Infectious bronchitis virus (IBV) |
| Applications： | For passage in eggs, clarify sample and use supenatant fluid for inoculum via allantoic cavity. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Specimen from chicks with naturally occurring disease, New Jersey, 1935 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Reported to cause syncytial CPE in CEK TC with subsequent necrosis of cells. For passage in eggs, clarify sample and use supenatant fluid for inoculum via allantoic cavity. Chill eggs for harvest when one or two enbryos are dead and the remainder are week or sluggish. |
| Effect on Host： | Yes, in vivo effects: death of chicken embryo Death of CE Yes, in vitro effects: syncytia in chicken embryo kidney tissue culture |
| Recommended Host： | 10 day chicken embryo, i.a. inoculation Host of Choice: CE (10 day, i.a.) Host Range: CE, CAM and CEK TC chicken embryo (chorioallantoic membrane); chicken embryonic kidney cells in tissue culture |
| Growth Conditions： | Duration: 1-2 days at 37C, via allantoic route |
| Name of Depositor： | CH Cunningham |
| Special Collection： | ATCC |
| Source： | Specimen from chicks with naturally occurring disease, New Jersey, 1935 |
| Year of Origin： | 1935 |
| References： | Cunningham CH. . Adv. Vet. Sci. 14: 106-148, 1970. McIntosh K. . Curr. Top. Microbiol. Immunol. 63: 86-119, 1974. Beaudette FR. . J. Am. Vet. Med. Assoc. 90: 51, 1937. |