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| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS213314 |
| Classification： | Poxviridae, Orthopoxvirus |
| Deposited As： | Vaccinia virus, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | IHD-W |
| Applications： | IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. The host range includes most mammalian cells and tissues. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | The IHD-W subtype was derived in Japan from IHD-J strain of
vaccinia by selection for the ability to induce distinct clear plaques in culture. |
| Product Format： | frozen |
| Storage Conditions： | -70°C |
| Comments： | IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from the IHD-J parent strain, in that IHD-W is unable to induce an active hemagglutinin in the plasma membrane and does elicit polykaryoctosis. The host range includes most mammalian cells and tissues. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from IHD-J in that IHD-W is HA(-) and IHD-J is HA(+). -70C |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects in tissue culture CPE |
| Recommended Host： | L2 cells (ATCC CCL-149); chicken embryo fibroblasts Host of Choice: L2 and CEF Host Range: most mammalian cells and tissues most mammalian cells and tissues |
| Growth Conditions： | Duration: 1-5 days at 37C |
| Cryopreservation： | Storage temperature: -70C |
| Name of Depositor： | S Dales |
| Special Collection： | NCRR Contract |
| Source： | The IHD-W subtype was derived in Japan from IHD-J strain of
vaccinia by selection for the ability to induce distinct clear plaques in culture. |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 |