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微信小章| 产品名称： | Chlamydia muridarum Everett et al. |
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| 商品货号： | TS213321 |
| Classification： | Chlamydiaceae |
| Deposited As： | Chlamydia trachomatis (Busacca) Rake |
| Agent： | Chlamydia muridarum Everett et al. |
| Strain： | Mouse Pneumonitis strain Nigg II |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Although this agent is not known to be pathogenic for man, it should be handled with care. Mice receiving 1:100 dilution i.n. of infected y.s. occasionally develop pneumonia and die.
The Nigg II strain was first deposited by F. B. Gordon as yolk-sac adapted material. It was subsequently redeposited as tissue culture adapted material by J. Schachter
This preparation has tested positive for Mycoplasma contamination Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies |
| Recommended Host： | McCoy (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: 48 hours at 37°C in McCoy cells (ATCC®xa0CRL-1696™).xa0Add glassbeads and vortex preparation to disrupt cells.xa0 Infect monolayer with disrupted material.xa0 Centrifuge at 3000 x rpm (750 x g) for 1 hour.xa0 Feed with fresh growth medium containing 1-2 µg/mL cycloheximide.xa0 Incubate at 37°C for 48 hours. Incubation: 48 hours |
| Name of Depositor： | FB Gordon, J Schachter |
| Source： | Normal mice, 1939 |
| Year of Origin： | 1939 |
| References： | Pal S, et al. Immunization with an acellular vaccine consisting of the outer membrane complex of Chlamydia trachomatis induces protection against a genital challenge. Infect. Immun. 65: 3361-3369, 1997. PubMed: 9234798 Nigg C. An unidentified virus which produces pneumonia and systemic infection in mice. Science 95: 49, 1942. Pal S, et al. Intranasal immunization induces long-term protection in mice against a Chlamydia trachomatis genital challenge. Infect. Immun. 64: 5341-5348, 1996. PubMed: 8945586 Kaushic C, et al. Chlamydia trachomatis infection in the female reproductive tract of the rat: influence of progesterone on infectivity and immune response. Infect. Immun. 66: 893-898, 1998. PubMed: 9488372 Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462 type strain |
| Cross References： | Nucleotide (GenBank) : AJ012177 Chlamydia muridarum partial rnpB gene for RNase P RNA subunit. |