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| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS213328 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | rVV-hgp100 |
| Applications： | VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI. |
| Effect on Host： | Cell enlargement and clustering of rounded cells in cell culture; plaques with rounding and eventual sloughing. |
| Recommended Host： | Production Host: Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts)). Production Host: xa0Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts).) Alternate Hosts: BS-C-1 cells (ATCC CCL-26), Vero cells (ATCC CCL-81), and HeLa cells (ATCC CCL-2) |
| Growth Conditions： | Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Process virus by freeze/ thawing it three times by vapor phase of liquid nitrogen and 37.0°C water bath (vortex mixing on high speed for 20 seconds between each freeze/ thaw), sonicating with 40% power for 30 seconds, and then a final vortex mixing on high speed for 30 seconds. Remove cell growth medium from the flask, and inoculate with a small volume of virus stock diluted in virus growth medium to provide a MOI of about 1-3. Adsorb 1-2 hours at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubate infected culture for 1-3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor： | Dr. Zhiya Yu from Surgery Branch/ NCI/ NIH |
| Source： | rVV-hgp100 was constructed in Dr. Nicolas P. Restifos laboratory of Surgery Branch/ NCI/ NIH in the early 1990s. |