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| 产品名称： | Felid herpesvirus 1 |
|---|---|
| 商品货号： | TS213360 |
| Classification： | Herpesviridae, Varicellovirus |
| Deposited As： | Feline rhinotracheitis virus |
| Agent： | Felid herpesvirus 1 |
| Strain： | FVRm (modified) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation from primary cell culture product ATCC® VR-815™ to CRFK cells (ATCC® CCL-94™).xa0 ATCC® VR-815™ and ATCC® VR-1787™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. Item is a vaccine strain. Antigenicity is specific for virus. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | CRFK (ATCC® CCL-94™) |
| Growth Conditions： | Temperature: 31°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 31°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-5 days |
| Name of Depositor： | EV Davis, Norden Laboratories, Inc. |
| Source： | Throat swab from a 3 month old cat |
| References： | Davis E. Feline viral rhinotracheitis vaccine and combination feline viral rhinotracheitis-calicivirus vaccine prepared therefrom. US Patent 4,031,204 dated Jun 21 1977 |