| 产品名称: | Human herpesvirus 2 |
|---|---|
| 商品货号: | TS213372 |
| Classification: | Herpesviridae, Simplexvirus |
| Deposited As: | Herpes simplex virus 2 |
| Agent: | Human herpesvirus 2 |
| Strain: | MS |
| Common Name: | Herpes simplex 2 |
| Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format: | frozen 1 mL per vial |
| Storage Conditions: | -70°C or colder |
| Images: | |
| Comments: | Deposited as Herpes simplex virus 2. |
| Effect on Host: | CPE, cell rounding, clumping, and sloughing |
| Recommended Host: | Vero (ATCC® CCL-81™) |
| Growth Conditions: | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: 2-5 days |
| Name of Depositor: | WR Dowdle, K Herrmann, Centers for Disease Control and Prevention |
| Special Collection: | NCRR Contract |
| Source: | Brain of a 50 year old female with multiple sclerosis, Iceland, 1961 |
| References: | Dowdle WR, et al. Association of antigenic type of herpesvirus hominis with site of viral recovery. J. Immunol. 99: 974-980, 1967. PubMed: 4295116 Gudnadottir M, et al. Virus isolated from the brain of a patient with multiple sclerosis. Exp. Neurol. 9: 85-95, 1964. PubMed: 14126126 Pauls FP, Dowdle WR. A serologic study of herpesvirus hominis strains by microneutralization tests. J. Immunol. 98: 941-947, 1967. PubMed: 4290495 |