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微信小章| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS213373 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts19 |
| Applications： | Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection. |
| Biosafety Level： | 2 BSL 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This conditional lethal mutant, originally designated plaque isolate no. 2096, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance without membranes in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | This conditional lethal mutant, originally designated plaque isolate no. 2096, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance without membranes in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses |
| Effect on Host： | Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions： | Duration: 1-3 days at 31 C with 5% CO2 |
| Name of Depositor： | S Dales, R. Condit |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |