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| 产品名称： | Murine leukemia virus |
|---|---|
| 商品货号： | TS213399 |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| Agent： | Murine leukemia virus |
| Strain： | AKR 13 MCF |
| Applications： | Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | AKR/J mouse (thymus) co-cultivated with Mv-1 Lu cells (ATCC CCL 64) |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. This preparation has tested positive for A. laidlawii. Typical retrovirus: buoyant density 1.16 g/cc in sucrose, lipid-containing envelope, infectivity rapidly destroyed by freezing and thawing; neutralized by antiserum to both xenotropic and AKR ecotropic viruses; possesses gs-1 antigen of MuLV. |
| Effect on Host： | tumorigenicity: Accelerated leukemia in AKR mice no CPE in Mv-1 Lu cells.xa0 Assay in MiCl cells (ATCC CCL-64.1), produces CPE foci |
| Recommended Host： | Mv 1 Lu (mink lung) cells (ATCC CCL-64) Host of Choice: Mv-1 Lu cells (ATCC CCL-64) Host Range: AKR mouse, Mv-1 Lu cells, MiCl cells AKR mouse; Mv 1 Lu cells (ATCC CCL-64) |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JW Hartley |
| Source： | AKR/J mouse (thymus) co-cultivated with Mv-1 Lu cells (ATCC CCL 64) |
| References： | Elder JH, et al. Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc. Natl. Acad. Sci. USA 74: 4676-4680, 1977. PubMed: 200928 |