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微信小章| 产品名称： | Human herpesvirus 1 |
|---|---|
| 商品货号： | TS213492 |
| Classification： | Herpesviridae, Simplexvirus |
| Deposited As： | Herpes simplex virus 1 |
| Agent： | Human herpesvirus 1 |
| Strain： | F |
| Common Name： | Herpes simplex virus 1 |
| Applications： | Susceptibility testing Testing virucides |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Comments： | Deposited as Herpes simplex virus 1. This strain exhibits slight cross reactivity with certain Human herpesvirus 2 monoclonals. |
| Effect on Host： | CPE, refractile rounding and cell clumping |
| Recommended Host： | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions： | Temperature: 34°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: 1-3 days |
| Name of Depositor： | B Roizman, Institut Merieux |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | NCRR Contract |
| Source： | Facial vesicle |
| References： | Antiviral Susceptibility Testing: Herpes Simplex Virus by Plaque Reduction Assay; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M33-A. ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104 Frankel N, Roizman B. Herpes simplex virus: genome size and redundancy studied by renaturation kinetics. J. Virol. 8: 591-593, 1971. PubMed: 4331657 Kieff ED, et al. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J. Virol. 8: 125-132, 1971. PubMed: 4329966 Kieff ED, et al. Genetic relatedness of type 1 and type 2 herpes simplex viruses. J. Virol. 9: 738-745, 1972. PubMed: 4337161 Roizman B. Perspectives in Virology VIII. New York, NY: Academic Press; . |