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微信小章| 产品名称： | Canine parainfluenza virus |
|---|---|
| 商品货号： | TS213511 |
| Classification： | Paramyxoviridae, Paramyxovirinae, Unclassified |
| Agent： | Canine parainfluenza virus |
| Strain： | 78-238 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | This an encephalitogenic strain of Canine parainfluenza virus. |
| Effect on Host： | Yes, in vitro effects: cytopathic effect, syncytial sloughing CPE, syncytial sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
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| Recommended Host： | Recommended Host:xa0 ATCC® CCL-81x99 (Vero) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 36.0°C Duration: 3-4 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | ATCC, JF Evermann |
| Special Collection： | ATCC |
| Source： | derived from existing strain |
| References： | Evermann JF, et al. Isolation of a paramyxovirus from the cerebrospinal fluid of a dog with posterior paresis. J. Am. Vet. Med. Assoc. 177: 1132-1134, 1980. PubMed: 6254938 Evermann JF, et al. Properties of an encephalitogenic canine parainfluenza virus. Arch. Virol. 68: 165-172, 1981. PubMed: 6168243 or colder ATCC VR-963 was filtered to remove bacterial and fungal contaminants and reaccessioned as TS213511. ATCC VR-963 was isolated from cerebrospinal fluid from dog with posterior paresis. or colder |