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微信小章| 产品名称： | Adeno-associated virus 2 |
|---|---|
| 商品货号： | TS213542 |
| Classification： | Parvoviridae, Dependovirus, Adeno-associated virus-2 |
| Deposited As： | Adeno-associated virus type 2 |
| Agent： | Adeno-associated virus 2 |
| Strain： | H |
| Applications： | Resistant to heat 56°C 4 hours; this property can be used to selectively inactivate the helper adenovirus - Human adenovirus 2 (ATCC® VR-846™)xa0
Cross-reacts with AAV-3; no hemagglutinin detected |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： |
Depends upon helper virus Human adenovirus 2 ATCC® VR-846™ for replication.xa0 Helper virus may be inactivated at 56°C for 30 minutes. Resistant to heat 56°C 4 hours; this property can be used to selectively inactivate the helper adenovirus. Resistant to ether and chloroform. Cross-reacts with AAV-3; no hemagglutinin detected. Depends upon helper adenovirus for replication. |
| Effect on Host： | Adenovirus CPE, refractile rounding and cell sloughing.xa0 AAV shows no CPE. |
| Recommended Host： | HEK-293 cells (ATCC® CRL-1573™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus and helper virus(e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1.0 each for virus and helper virus). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until helper virus CPE is progressed through 80-90% of the monolayer. AAV does not cause CPE. |
| Name of Depositor： | MD Hoggan |
| Source： | Adenovirus type 12 strain 97838 in KB cells |
| References： | Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666 |