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| 产品名称： | Encephalomyocarditis virus |
|---|---|
| 商品货号： | TS213544 |
| Classification： | Picornaviridae, Cardiovirus |
| Agent： | Encephalomyocarditis virus |
| Strain： | M-variant (TC passaged) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | ATCC® VR-1479™ (M-varient)xa0is a cell culture passaged linage of the M-varient (ATCC® VR-1314™) reported to induce diabetes in selected strains of mice such as Balb/cBy and DBA/2 mice. Note that virus loses diabetagenic properties upon multiple passage in cell culture. |
| Effect on Host： | Rounding and sloughing with general degenerationxa0 |
| Recommended Host： | NCTC Clone 929 (ATCC® CCL-1™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 75-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days |
| Name of Depositor： | ATCC |
| Special Collection： | NCRR Contract |
| Source： | ATCC®xa0VR-1479™xa0was created by serially passaging ATCC®xa0VR-1314™xa0two times onto NCTC clone 929 (L-929) cells, ATCC®xa0CCL-1™. The original strain was isolated from the heart of mice inoculated by peripheral route with EMC of swine. |
| References： | Craighead JE. Pathogenicity of the M and E variants of the encephalomyocarditis (EMC) virus. I. Myocardiotropic and neurotropic properties. Am. J. Pathol. 48: 333-345, 1966. PubMed: 4285783 |