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微信小章| 产品名称： | Bovine herpesvirus 4 |
|---|---|
| 商品货号： | TS213546 |
| Classification： | Herpesviridae, Rhadinovirus |
| Deposited As： | Bovine herpesvirus |
| Agent： | Bovine herpesvirus 4 |
| Strain： | DN-599 |
| Applications： | Produces respiratory illness in calves |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Serologically distinct from infectious bovine rhinotracheitis, bovine mammilitis, malignant catarrhal fever, pseudorabies allerton and Movar type herpesvirus. Produces respiratory illness in calves. |
| Effect on Host： | CPE, refractile and rounded cells |
| Recommended Host： | BT (ATCC® CRL-1390™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are approximately 95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-4 days |
| Name of Depositor： | SB Mohanty, University of Maryland, College Park, Maryland |
| Source： | Nasal swabs from 1 ½ year old steer with clinical symptoms of respiratory disease, Maryland |
| References： | Mohanty SB, et al. A new bovine herpesvirus and its effect on experimentally infected calves. Brief report. Arch. Gesamte Virusforsch. 33: 394-395, 1971. PubMed: 4107037 |