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| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS213567 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | CL |
| Applications： | This strain was used by J. Craigie in studies of antigenic composition and by T. M. Rivers and collaborators in studies of chemical composition of vaccinia virus. It is a dermal strain and produces rather small, opaque, white pocks on chorioallantoic membranes. |
| Biosafety Level： | 2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (Laboratory safety is discussed in the current publication of the Biosafety in Microbiological and Biomedical Laboratories, from the U.S. Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes for Health)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Probably derived from New York City Department of Health strain of vaccinia |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | REFERENCE STRAIN Ref   Fenner, F and Burnet, FM. A short description of the Poxvirus group (Vaccinia and related viruses). Virology 4: 305-314, 1957. PubMed: 13496547. This strain was used by J. Craigie in studies of antigenic composition and by T. M. Rivers and collaborators in studies of chemical composition of vaccinia virus. It is a dermal strain and produces rather small, opaque, white pocks on chorioallantoic membranes. It is non-pathogenic for mice (i. c.). The virus is very resistant and may contaminate the laboratory. |
| Effect on Host： | Cell culture: CPE is refractile cell rounding. CPE is refractile cell rounding in culture. In vivo: Causes pocks on chorioallantoic membrane. |
| Recommended Host： | Production Host: LLC-MK2 Derivative cells (ATCC CCL-7.1) Alternate Host: Chicken embryo chorioallantoic membrane (CAM) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 90-95% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 7-10 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 1-3 days at 37°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | F. Fenner; Australian National University |
| Chain of Custody： | ATCC |
| Source： | Probably derived from New York City Department of Health strain of vaccinia |
| References： | Fenner, F and Burnet, FM. A short description of the Poxvirus group (Vaccinia and related viruses). Virology 4: 305-314, 1957. PubMed: 13496547. Craigie J. . Br. J. Exp. Pathol. 13: 259, 1932. Parker RF, et al. Further studies of the infectious unit of Vaccinia. J. Exp. Med. 74: 263-281, 1941. |