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购买须知
| 产品名称： | Mumps rubulavirus |
|---|---|
| 商品货号： | TS213593 |
| Classification： | Paramyxoviridae, Rubulavirus |
| Deposited As： | Mumps |
| Agent： | Mumps rubulavirus |
| Strain： | Enders |
| Common Name： | Mumps virus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Same strain as VR-106. Adapted at CDC to grow in continuous cell line NCI-H292 (HuT-292)--see Castells et al. Arch Virol. 115:277-288, 1990. After adsorbing virus, feed with medium containing trypsin but NO serum. |
| Effect on Host： | Cytopathic effects in tissue culture. No CPE or extremely subtle CPE, cells thicken and some clumping. Hemadsorption and hemagglutination with chicken or guinea pig red blood cells. |
| Recommended Host： | NCI-H292 (ATCC® CRL-1848™) |
| Growth Conditions： | Temperature: 36°C
Atmosphere: 5% CO2 in air recommended
Incubation: 6 to 12 days at 36°C rocking, a 5% CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 48 to 72 hours old and 100% confluent. |
| Name of Depositor： | JC Hierholzer |
| Source： | Pooled saliva from patients, Massachusetts |
| References： | Enders JF, et al. Immunity in mumps: I. Experiments with monkeys (Macacus mulatta). The development of complement-fixing antibody following infection and experiments on immunization by means of inactivated virus and convalescent human serum. J. Exp. Med. 81: 93-117, 1945. PubMed: 19871448. Levens JH, Enders JF. . Science 102: 117, 1945. |