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微信小章| 产品名称： | Feline infectious peritonitis virus |
|---|---|
| 商品货号： | TS213597 |
| Classification： | Coronaviridae, Alphacoronavirus, Alphacoronavirus 1 |
| Agent： | Feline infectious peritonitis virus |
| Strain： | DF2 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Comments： | Cross reacts with canine coronavirus, TGE and human 229B. |
| Effect on Host： | CPE, Cell rounding and degeneration |
| Recommended Host： |
CRFK cells (ATCC® CCL-94™)
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| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa03-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | EV Davis |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | ATCC |
| Source： | Spleen of cat with typical FIP disease |
| References： | Evermann JF, et al. Characterization of a feline infectious peritonitis virus isolate. Vet. Pathol. 18: 256-265, 1981. PubMed: 7467085 McKeirnan AJ et al. Comparative properties of feline coronaviruses in vitro. Can. J. Vet. Res. 51(2): 212-216, 1987. PubMed: 1255305 |