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| 产品名称： | Human coronavirus 229E |
|---|---|
| 商品货号： | TS213598 |
| Classification： | Coronaviridae, Coronavirus |
| Agent： | Human coronavirus 229E |
| Strain： | 229E |
| Common Name： | Human coronavirus |
| Applications： | No hemolysis production. Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C. Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). RU1 are human embryonic lung fibroblasts developed by CDC |
| Effect on Host： | CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer. |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 35°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended. |
| Name of Depositor： | HS Kaye |
| Special Collection： | ATCC |
| Source： | Nasal and throat swabs from man with mild upper respiratory illness |
| References： | Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768 |