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| 产品名称： | Nuclear polyhedrosis virus, Autographa californica |
|---|---|
| 商品货号： | TS213599 |
| Classification： | Baculovirus |
| Deposited As： | Nuclear Polyhedrosis Virus, Lymantria dispar (plaque purified) |
| Agent： | Nuclear polyhedrosis virus, Autographa californica |
| Strain： | LdMNPV-Ab-a624 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA |
| Product Format： | freeze-dried |
| Storage Conditions： | -20°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | To propagate, rehydrate in water (or thaw), and feed to gypsy moth larvae (and collect hemolymph), or dissolve in 0.1 M Na2CO3, 0.2 M NaCl (pH 10.8) and inoculate gypsy moth cell culture (and collect cell lysate and supernate). |
| Effect on Host： | Yes, in vivo effects: Kills 4th-5th instar Lymantria dispar larvae, 5-7 days Yes, in vitro effects: nuclear swelling and inclusion bodies in cell culture, 3-4 days |
| Recommended Host： | gypsy moth larvae gypsy moth larvae and closely related lymantrids; IPLB LDFB, IPLB 652Y, and IPLB EIT cell cultures |
| Growth Conditions： | Temperature: 28°C Incubation: 7 days 7 days |
| Name of Depositor： | M Shapiro |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA |
| References： | Shapiro M, et al. More virulent biotype isolated from wild-type virus. US Patent 5,132,220 dated Jul 21 1992 |