微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Rickettsia argasii |
|---|---|
| 商品货号： | TS213609 |
| Classification： | Rickettsiaceae, Rickettsia |
| Deposited As： | Rickettsia argas |
| Agent： | Rickettsia argasii |
| Strain： | T170-B |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | New Taxon. It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects. |
| Effect on Host： | No CPE produced |
| Recommended Host： | Vero cells (ATCC CCL-81) |
| Growth Conditions： | Temperature: 35°C Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and inoculate with an appropriate volume of Rickettsiae (e.g. 1 mL per 25 cm2) diluted in agent growth medium to provide an appropriate MOI (e.g. MOI of 50 for 50 Rickettsiae/ cell). Adsorb 1 hour at 800 x g at 20°C in a low speed centrifuge in closed buckets. End adsorption by adding agent growth medium to culture(s). Incubation: Incubate infected culture(s) for 7-14 days at 35°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | L Izzard |
| Source： | Tissue from the bat tick Argas dewae (soft tick), Southern Victoria, Australia. |