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| 产品名称： | Vaccinia virus |
|---|---|
| 商品货号： | TS213614 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Vaccinia virus |
| Strain： | vTF7-3 Wr |
| Applications： | Expresses bacteriophage T7 RNA polymerase. Expresses bacteriophage T7 RNA polymerase. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Constructed in vitro |
| Product Format： | frozen |
| Storage Conditions： | -70°C |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | Cells which have been infected with this construct can be transfected by plasmids which contain a gene targeted for expression inserted between a T7 promoter and T7 terminator. Expresses bacteriophage T7 RNA polymerase. Recombinant virus constructed in vitro. Expresses bacteriophage T7 RNA polymerase. Expression of the T7 RNA polymerase is directed by the P7.5 promoter which includes early and late regulatory signals. Constructed by transfecting vaccinia virus-infected cells with pTF703 (ATCC 67202). |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (plaque formation) on BS-C-1 and CV-1 monolayers CPE, rounding, stringing, and cell destruction |
| Name of Depositor： | NIH, B Moss, NIH |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Constructed in vitro |
| References： | Fuerst TR, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83: 8122-8126, 1986. PubMed: 3095828 Zheng Z, et al. Mutations in the carboxyl-terminal hydrophobic sequence of human cytomegalovirus glycoprotein B alter transport and protein chaperone binding. J. Virol. 70: 8029-8040, 1996. PubMed: 8892927 |