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| 产品名称： | Canine distemper virus |
|---|---|
| 商品货号： | TS213615 |
| Classification： | Paramyxoviridae, Morbillivirus |
| Deposited As： | Canine distemper |
| Agent： | Canine distemper virus |
| Strain： | Lederle |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Belongs to the same virus subgroup as measles and rinderpest. |
| Effect on Host： | CPE, refractile cell rounding and degeneration |
| Recommended Host： | SL-29 (ATCC® CRL-1590™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 5-8 days. Replace virus growth medium every 2-3 days. |
| Name of Depositor： | VJ Cabasso, PJ Vasington, Lederle Laboratories Division of American Cyanamid Company |
| Source： | Field case of distemper encephalitis in dog |
| References： | Cabasso VJ, Cox HR. A distemper-like strain of virus derived from a case of canine encephalitis: its adaptation to the chick embryo and subsequent modification. Cornell Vet. 42: 96-107, 1952. PubMed: 14905890 |