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| 产品名称： | Human rhinovirus 16 |
|---|---|
| 商品货号： | TS213659 |
| Classification： | Picornaviridae, Enterovirus |
| Deposited As： | Rhinovirus 16 |
| Agent： | Human rhinovirus 16 |
| Strain： | 11757 |
| Common Name： | Rhinovirus 16, HRV-16 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | The pH of the medium should be maintained in a range of 7.0-7.3. Rocking of cultures during adsorption may enhance viral replication and CPE. Deposited as Rhinovirus HRV-16. HA is not demonstrated. Not inactivated by ether. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | H1-HeLa (ATCC® CRL-1958™) |
| Growth Conditions： | Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while continuously rocking. End adsorption by adding virus growth medium.
Incubation: 1-7 days in a humidified 5% CO2 atmosphere until 90% of the culture shows CPE. |
| Name of Depositor： | RM Conant, VV Hamparian, The Ohio State University |
| Special Collection： | ATCC |
| Source： | Throat swab from healthy 2-year-old female, Washington, DC, 1960 |
| References： | Hamparian VV, Conant RM. Rhinoviruses: a numbering system. Nature 213: 761-763, 1967. PubMed: 4291698 Johnson KM, Rosen L. Characteristics of five newly recognized Enteroviruses recovered from the human oropharynx. Am. J. Epidemiol. 77(1): 15-25, 1963. |