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| 产品名称： | Dengue virus type 2 |
|---|---|
| 商品货号： | TS213696 |
| Classification： | Flaviviridae, Flavivirus, Dengue virus |
| Agent： | Dengue virus type 2 |
| Strain： | New Guinea C |
| Applications： | Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Derived by adaptation from in vivo product ATCC® VR-1255™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1255™ and ATCC® VR-1584™ have not been compared for sequence or infectivity in in vivo and tissue culture. |
| Effect on Host： | CPE, cell aggregation, sloughing, death |
| Recommended Host： | LLC-MK2 Derivative (ATCC® CCL-7.1™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 6-8 days |
| Name of Depositor： | CL Wisseman, University of Maryland School of Medicine; Yale Arbovirus Research Unit; National Institute of Allergy and Infectious Diseases; ATCC® |
| Source： | Serum of febrile patient, New Guinea, 1944 |
| References： | Sabin AB. Research on dengue during World War II. Am. J. Trop. Med. Hyg. 1: 30-50, 1952. PubMed: 14903434 |