微信小陈
微信小章| 产品名称： | H-1 parvovirus |
|---|---|
| 商品货号： | TS213731 |
| Classification： | Parvoviridae, Parvovirinae, Parvovirus |
| Deposited As： | H-1 virus |
| Agent： | H-1 parvovirus |
| Strain： | Toolan |
| Applications： | Produces deformity or death in newborn hamsters and neutralizing antibodies in humans, monkeys, hamsters and rats. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | xa0-70°C or colder |
| Genome Sequenced Strain： | Parvovirus h-1, complete genome |
| Comments： | Plaque purified on Salk MkHe.xa0 Hemagglutinates a variety of RBC, particularly guinea pig, hamster and human, and also has a CF antigen.xa0 Produces deformity or death in newborn hamsters and neutralizing antibodies in humans, monkeys, hamsters and rats. |
| Effect on Host： | Cytopathic effects (cell death) in tissue culture; death or deformity of newborn hamster depending on virus concentration |
| Recommended Host： |
Chang L cells (ATCC® CCL-13™)
|
| Growth Conditions： | Temperature:xa037°C Recommendations for Infection:xa0xa0Plant cells 24-48 hours in advance and infect when cultures are about 70% confluent.xa0xa0 Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1.xa0 Adsorb 1-2 hours at 37°C in a humidified 5% CO2xa0atmosphere, rocking every 20-30 minutes to redistribute inoculum.xa0 End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 5 days at 37°C temperature in a humidified 5% CO2xa0atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | HW Toolan |
| Source： | Human embryo lung (4 months operative abortion) from mother with metastatic breast cancer, who had an HAI and neutralizing titer of 1:640 to H-1 virus. |
| References： | Toolan H, Ledinko N. Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208: 812-813, 1965. Pubmed: 4958078 Karasaki S. Size and ultrastructure of the H-viruses as determined with the use of specific antibodies. J Ultrastruct Res 16(1): 109-122, 1966. Pubmed: 4959542 Proc. Am. Assoc. Cancer Res. 5: 64, 1964. Rhodes SL, Paradiso PR. Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation. J Virol 45(1): 173-184, 1983. |
| Cross References： | Nucleotide (GenBank) : X01457.1 Parvovirus h-1, complete genome |