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微信小章| 产品名称： | Human adenovirus 5 |
|---|---|
| 商品货号： | TS213733 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus C |
| Deposited As： | Adenovirus type 5 |
| Agent： | Human adenovirus 5 |
| Strain： | Adenoid 75 |
| Applications： | Viruscide testing Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Not inactivated by ether. Contains soluble group-specific complement fixation antigen. Agglutinates rat red blood cells incompletely. |
| Effect on Host： | CPE, refractile rounding, clumping and sloughing |
| Recommended Host： | HeLa (ATCCxa0CCL-2) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-3 days |
| Name of Depositor： | RJ Huebner, National Institutes of Health; M Shaffer, Public Health Research Institute of the City of New York |
| Special Collection： | NCRR Contract |
| Source： | Spontaneously degenerating tissue culture of adenoid tissue from a 4-year-old girl with enlarged chronically infected tonsils, Washington DC, 1953 |
| References： | Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006. Chemical disinfectants and antiseptics --- Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 14476:2005. Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Huebner RJ, et al. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. Exp. Biol. Med. 84: 570-573, 1953. PubMed: 13134217 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955. |