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微信小章| 产品名称： | Porcine parvovirus NADL-2 |
|---|---|
| 商品货号： | TS213739 |
| Classification： | Parvoviridae, Parvovirus, Porcine parvovirus |
| Deposited As： | Porcine parvovirus |
| Agent： | Porcine parvovirus NADL-2 |
| Strain： | NADL-2 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | At the time this deposit was made, TC of other species had not been extensively evaluated as viral hosts. HA activity in chicken, rat, cat, and GP RBC at 22°C and 4°C (HA activity is four fold higher at 4°C than at 22°C for cat RBC). No HA activity in pig, hamster, sheep or cattle RBC. Virus must be freeze-thawed 3 times before inoculating and/or passing with fresh cells. CPE most pronounced when cells are exposed to the virus just before or after subculturing. |
| Effect on Host： | CPE, nuclear inclusions and degeneration |
| Recommended Host： | ST (ATCC® CRL-1746™) |
| Growth Conditions： | Temperature 37°C Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 30-50% confluency. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 1:100 dilution) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation. Incubation: 3 -5 days |
| Name of Depositor： | WL Mengeling |
| Source： | Porcine leukocytes from blood samples collected at an abattoir |
| References： | Mengeling WL. Porcine parvovirus: properties and prevalence of a strain isolated in the United States. Am. J. Vet. Res. 33: 2239-2248, 1972. PubMed: 4628211 |