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| 产品名称： | Human Coxsackievirus B4 |
|---|---|
| 商品货号： | TS213743 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus B |
| Deposited As： | Coxsackievirus B 4 |
| Agent： | Human Coxsackievirus B4 |
| Strain： | J.V.B. (Benschoten) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Stool from 10-year-old child, New York, 1951 |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： |
LLC-MK2 Derivative (ATCC® CCL-7.1™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | H Plager |
| Source： | Stool from 10-year-old child, New York, 1951 |
| Year of Origin： | 1951 |
| References： | Dalldorf G. The Coxsackie viruses. Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582 Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture. Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332 |