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| 产品名称： | Murine hepatitis virus |
|---|---|
| 商品货号： | TS213766 |
| Classification： | Nidovirales, Coronaviridae, Coronavirus,Group 2 |
| Agent： | Murine hepatitis virus |
| Strain： | MHV-S Log #3239/17 |
| Common Name： | Murine hepatitis virus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Isolated by i.c. and i.p. passage of viscera of 8 sick CD-1 mice in infant and suckling mice |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Highly infectious in susceptible mouse colonies. Despite high mortality, the clinical signs in infected mice are often non-specific, with weakness or are suggestive of encephalitis with spasticity. Necropsy occasionally reveals small yellow or whitish spots on the liver. Highly infectious in susceptible mouse colonies. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects in tissue culture CPE, apoptosis and slough off in clumps |
| Recommended Host： | NCTC clone 1469 cells (ATCC CCL-9.1) Recommended Host: NCTC clone 1469 cells (ATCC® CCL-9.1x99) newborn mouse; NCTC clone 1469 cells (ATCC CCL-9.1) |
| Growth Conditions： | Temperature: 37°C
Incubation: 18 to 48 hours at 37°C, a 5% CO2 in air atmosphere is recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent). |
| Name of Depositor： | MJ Collins |
| Special Collection： | ATCC |
| Source： | Isolated by i.c. and i.p. passage of viscera of 8 sick CD-1 mice in infant and suckling mice |
| References： | Hartley JW, et al. Mouse hepatitis virus infection as a highly contagious, prevalent, enteric infection of mice. Proc. Soc. Exp. Biol. Med. 112: 161-165, 1963. PubMed: 13982823 |