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微信小章| 产品名称： | Fowl adenovirus 3 |
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| 商品货号： | TS213768 |
| Classification： | Adenoviridae, Aviadenovirus, Fowl adenovirus D |
| Deposited As： | Avian adenovirus Type 3 |
| Agent： | Fowl adenovirus 3 |
| Strain： | IBH-2A (Tipton) |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-828™ to LMH cells (ATCC® CRL-2117™). ATCC® VR-828™ and ATCC® VR-1854™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. |
| Effect on Host： | CPE, refractile rounding and cell degeneration |
| Recommended Host： | LMH cells (ATCC® CRL-2117™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 5-8 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | BW Calnek, Cornell University |
| Source： | Liver of chicken with aplastic anemia and inclusion body hepatitis |
| References： | Calnek BW, Cowen BS. Adenoviruses of chickens: serologic groups. Avian Dis. 19: 91-103, 1975. PubMed: 1120041 Fadly AM, Winterfield RW. Isolation and some characteristics of an agent associated with inclusion body hepatitis, hemorrhages, and aplastic anemia in chickens. Avian Dis. 17: 182-193, 1973. PubMed: 4633275 |