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微信小章| 产品名称： | Enterovirus D68, High titer |
|---|---|
| 商品货号： | TS213770 |
| Agent： | Enterovirus D68, High titer |
| Strain： | US/KY/14-18953 |
| Applications： | Virucide testing Respiratory research In vitro infectivity assay Antiviral studies Spiking studies ELISpot assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 0.5 mL per vial Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | ATCC® VR-1825PQ™ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC® VR-1825™ via concentration through a sucrose cushion. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | RD cells (ATCC® CCL-136™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking continuously at 0.5 rpm. End adsorption by adding virus growth medium. Incubation: 2-3 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | Centers for Disease Control and Prevention |
| Source： | Nasopharyngeal swab from patient with respiratory illness, Kentucky, September 2014 |
| References： | Brown BA, et al. Seven Strains of Enterovirus D68 Detected in the United States during the 2014 Severe Respiratory Disease Outbreak. Genome Announc 2(6): e01201-14, 2014. PubMed: 25414503 |