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微信小章| 产品名称： | Zika virus, High titer |
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| 商品货号： | TS213772 |
| Classification： | Flaviviridae, Flavivirus |
| Deposited As： | Zika |
| Agent： | Zika virus, High titer |
| Strain： | PRVABC59 |
| Applications： | Vector-borne disease research In vitro infectivity assay Antiviral studies Spiking studies ELISpot assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 0.5 mL per vial Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Zika virus strain PRVABC59, complete genome |
| Images： |  |
| Comments： | ATCC®xa0VR-1843PQ™ is a high-titer, live virus suspended in culture medium. This product was prepared from ATCC®xa0VR-1843™ via concentration by centrifugal filtration. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero C1008 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 60-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | B Russell, Centers for Disease Control and Prevention |
| Source： | Human serum specimen, Puerto Rico, December 2015 |
| Year of Origin： | 2015 |
| References： | Dick GW, Kitchen SF, and Haddow AJ. Zika Virus. I. Isolations and Serological Specificity. Trans R Soc Trop Med Hyg 46: 509-520, 1952. PubMed: 12995440 Zhu Z, et al. Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg Microbes Infect 5: e22, 2016. |
| Cross References： | Nucleotide (GenBank) : KU501215 Zika virus strain PRVABC59, complete genome |